UV-Visible Spectroscopy

Ultraviolet-Visible Spectroscopy is a technique used for structure elucidation of organic compounds. This technique is majorly used for identifying conjugated dienes, polyenes, unsaturated carbonyl compounds and aromatic compounds. Aliphatic organic compounds do not show any absorption peak in this region. With the help of Woodward Fieser Rules, we can predict the absorption maximum of compounds showing UV-Visible light absorption. Beer-Lambert law suggests that as the concentration of compound under investigation increases absorption of UV-Visible light increases and it also increases if the length of sample tube (traveling path length) of light increases.